Effect of Nigella sativa on ischemia-reperfusion induced rat kidney damage

Effect of Nigella sativa on ischemia-reperfusion induced rat kidney damage

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Objective(s):There are a few previously reported studies about the effect of Nigella sativa oil on renal ischemia-reperfusion injury (IRI).The aim of the present study was to test the hypothesis whether pre- or post-treatment with N.sativa hydroalcoholic extract (NSE) would reduce tissue injury and oxidative damages in a berness white sneakers clinically relevant rat model of renal IRI.Materials and Methods: IRI was induced by clamping of bilateral renal arteries for 40 min fallowed by reperfusion for 180 min.

NSE was prepared in a Soxhlet extractor and administrated with doses of 150 mg/kg or 300 mg/kg at 1 hr before ischemia induction (P-150 and 300) or at here the beginning of reperfusion phase (T-150 and 300), via jugular catheter intravenously.The kidneys were then removed and subjected to biochemical analysis, comet assay or histopathological examination.Results: The kidneys of untreated IRI rats had a higher histopathological score (P.